The New Testament was written without the punctuation we are familiar with today, leaving modern readers with interpretative challenges. This article delves into how the absence of original punctuation in the scriptio continua style of Koine Greek demands careful scholarly work to discern the intended meaning of the biblical texts. By examining key passages, we uncover the profound impact punctuation has on our theological understanding and doctrinal conclusions. Inside this article, discover the profound layers of John 3:16 and how a single punctuation mark from modern translations can shift our understanding of one of the Bible's most pivotal messages of salvation.